Fig. 7: TLR4/MyD88/STAT1 upregulated lncRNA-F630028O10Rik. | Cell Death & Disease

Fig. 7: TLR4/MyD88/STAT1 upregulated lncRNA-F630028O10Rik.

From: TLR4 promotes microglial pyroptosis via lncRNA-F630028O10Rik by activating PI3K/AKT pathway after spinal cord injury

Fig. 7

a LncRNA-F630028O10Rik levels in LPS-treated cells following STAT1 silencing. **p < 0.01 versus Blank, #p < 0.05 versus LPS. b ChIP assay showing binding between STAT1 and lncRNA-F630028O10Rik. c LncRNA-F630028O10Rik and STAT1 levels in MyD88-knockdown cells. **p < 0.01. d, e Representative immunoblot and quantification of p-STAT1, STAT1, p-p65, p65 and MyD88 in each group. *p < 0.05, **p < 0.01. f STAT1 mRNA levels in WT-Sham, WT-SCI and TLR4−/−-SCI mice. *p < 0.05 versus WT-Sham, #p < 0.05 versus WT+SCI. g, h Representative immunoblot and quantification of p-STAT1, STAT1, p-p65, p65 and MyD88 in WT-Sham, WT-SCI and TLR4−/−-SCI mice. *p < 0.05 versus WT-Sham, #p< 0.05 versus WT+SCI. Data are the mean ± SD from 3 independent experiments.

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