Fig. 4: Downregulation PTPRM inhibits CCa proliferation, invasion, EMT, and lymphangiogenesis in vitro. | Cell Death & Disease

Fig. 4: Downregulation PTPRM inhibits CCa proliferation, invasion, EMT, and lymphangiogenesis in vitro.

From: High expression of PTPRM predicts poor prognosis and promotes tumor growth and lymph node metastasis in cervical cancer

Fig. 4: Downregulation PTPRM inhibits CCa proliferation, invasion, EMT, and lymphangiogenesis in vitro.The alternative text for this image may have been generated using AI.

a, b CCK8 assays revealed that the proliferation ability of SiHa and HeLa cells was significantly inhibited in the PTPRM knockdown cells. c–e Wound-healing assays were performed to investigate PTPRM knockdown suppressed migration of cervical cancer cells. Original magnification: ×100. f Transwell assays showed PTPRM knockdown impeded invasion of cervical cancer cells. Original magnification: ×100. g The morphology change of SiHa cells shown by TRITC phalloidin staining after PTPRM knockdown. Original magnification: ×400. h Western blot analysis of Snail, E-cadherin, N-cadherin, Vimentin and VEGF-C expression level in CCa cells of NC group and PTPRM knockdown group. i qRT-PCR confirmed VEGF-C expression level after PTPRM knockdown in CCa cells. j HLECs tube formation assay demonstrated the effect of PTPRM on lymphangiogenesis. Original magnification: ×200. Average length of tube formation was quantified. *, ** or ***: significantly different from the corresponding control, P < 0.05, P < 0.01 or P < 0.001, respectively.

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