Fig. 6: DOCK9-AS2 regulated proliferation, migration, invasion, EMT and stemness in PTC cells through Wnt/β-catenin pathway. | Cell Death & Disease

Fig. 6: DOCK9-AS2 regulated proliferation, migration, invasion, EMT and stemness in PTC cells through Wnt/β-catenin pathway.

From: Exosomal lncRNA DOCK9-AS2 derived from cancer stem cell-like cells activated Wnt/β-catenin pathway to aggravate stemness, proliferation, migration, and invasion in papillary thyroid carcinoma

Fig. 6

a CTNNB1 expression was evaluated by RT-qPCR in PTC cells transfected with sh-NC, sh-DOCK9-AS1#1, sh-DOCK9-AS2#1+miR-1972 inhibitor, sh-DOCK9-AS2#1+pcDNA3.1/SP1+miR-1972 inhibitor or sh-DOCK9-AS2#1+pcDNA3.1/CTNNB1. b Western blots of the downstream effectors (c-Myc, MMP2, MMP7, E-cad, and N-cad) in PTC cells of each group. c CCK-8 evaluated the viable PTC cells with indicated transfections. d Pictures and quantification of colonies generated by PTC cells with indicated transfections. e Pictures of invaded PTC cells under indicated transfections were taken and number of invasive cells were quantified in each field. Scale bar: 100 μm. f Pictures of scratch wound in PTC cells with wound width labeled. g Pictures and quantification of 3rd generation spheres formed by PTC cells with indicated transfection. Scale bar: 50 μm. h Western blot of stem cell specific genes in PTC cells of each group. *P < 0.05, **P < 0.01 n.s. meant no significance. Error bar denotes Mean ± S.D.

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