Fig. 2: Propofol suppresses H/R-induced autophagy. | Cell Death & Disease

Fig. 2: Propofol suppresses H/R-induced autophagy.

From: Propofol-induced MiR-20b expression initiates endogenous cellular signal changes mitigating hypoxia/re-oxygenation-induced endothelial autophagy in vitro

Fig. 2

a Built of the hypoxia/re-oxygenation model. The culture media was replaced with glucose and serum free DMEM. Then the HUVECs in hypoxic conditions was placed with 94% N2, 5% CO2, and 1% O2 using a small enclosed chamber filled at 37 °C for 12 h. Then the medium was changed to culture media and propofol was added with different concentrations: 25 μmol/L (P25), 50 μmol/L (P50), 100 μmol/L (P100), 150 μmol/L (P150) for 4 h in normal condition. b–e The expression of ULK1, Beclin1, and LC3 was determined in normal HUVECs, H/R injury HUVECs, H/R+DMSO HUVECs, and propofol post-hypoxia treatment HUVECs. f Autophagosomes and mitochondria were probed by anti-LC3 (green) and anti-TOM20 (red) in each group as above. Bar, 10 μm. Data were from three independent experiments. g–i PCR was performed to detect the expression of miR-20b, Beclin1, and ULK1 in normal HUVECs, H/R injury HUVECs, H/R+DMSO HUVECs, and propofol-treated HUVECs with the most effective concentration of 100 μmol/L. Data were mean ± SEM (n = 3); *p < 0.05, **p < 0.01, ***p < 0.001.

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