Fig. 4: The regulatory mechanism of miR-1307-mediated inhibition of PRRX1. | Cell Death & Disease

Fig. 4: The regulatory mechanism of miR-1307-mediated inhibition of PRRX1.

From: The rs7911488-T allele promotes the growth and metastasis of colorectal cancer through modulating miR-1307/PRRX1

Fig. 4

a The expression of PRRX1 protein in SW480-T and SW480-C cells. b The effect of miR-1307 on the expression of PRRX1 mRNA in SW480 and HCT-116 cells, which were respectively transfected with miR-1307 mimics or inhibitor for 48 h. c The effect of miR-1307 on the expression of PRRX1 protein in SW480 and HCT-116 cells, which were respectively transfected with miR-1307 mimics or inhibitor for 72 h. d The effect of miR-1307 on the expression activity of PRRX1/3′-UTR/pGL-3 constructs in SW480, CHO, and HEK293 cells. The upstream- and downstream-sequence of PRRX1 3′-UTR were amplified and then cloned into the pGL-3 plasmids. The PRRX1/3′-UTR/pGL-3 constructs were respectively transfected into SW480, CHO, and HEK293 cells with miR-1307 mimics. Data represent mean ± SD. Significance was assessed by two-sided t-test. ***P < 0.001; **P < 0.01; *P < 0.05.

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