Fig. 1: GADD45β expression levels increase in DF-1 cells following ALV-J infection. | Cell Death & Disease

Fig. 1: GADD45β expression levels increase in DF-1 cells following ALV-J infection.

From: ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy

Fig. 1

a DF-1 cells were infected with ALV-J for 24, 48, or 72 h and the expression levels of GADD45β were analyzed. Statistical differences were determined using two-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001. b Following 24, 48, or 72 h of ALV-J infection, total protein was extracted from DF-1 cells or CEF cells, the expression levels of GADD45β were analyzed using western blotting. The chart (b′) shows the quantification of GADD45β/β-actin in (b). c Cells were infected with ALV-J for 24 h and immunostained with rabbit anti-gp37 antibody (green). The expression levels of GADD45β (red) were analyzed using confocal microscopy. DAPI (blue) was used to stain the nuclear DNA. Scale bar, 10 μm. The chart (c′) shows the fluorescence intensity of endogenous GADD45β in (c). All experiments were repeated in triplicate.

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