Fig. 5: Hypoxia results in transient disruption of cardiomyocyte pri-miR-22 processing. | Cell Death & Disease

Fig. 5: Hypoxia results in transient disruption of cardiomyocyte pri-miR-22 processing.

From: Inhibition of the long non-coding RNA NEAT1 protects cardiomyocytes from hypoxia in vitro via decreased pri-miRNA processing

Fig. 5

a Pri-miR-22 processing reporter assay signal in iPS-CM grown in hypoxic conditions (1% pO2). ***p < 0.001 comparing the signal to baseline. b qRT-PCR analysis of pri-miR-22 and miR-22-3p expression in iPS-CM grown in hypoxic conditions. miR-22-3p and pri-miR-22 levels are expressed relative U6 RNA and GAPDH, respectively. Results are normalized to the mean of the baseline (0 h) samples. ***p < 0.001, **p < 0.01 comparing pri-miR-22 expression at time point 6 h and 24 h with time point 0 h. ##p < 0.01 comparing miR-22-3p expression at time point 6 h and 48 h with time point 0 h. All graphs show mean and standard deviation.

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