Fig. 4: DPP8/9 inhibitors induce pyroptosis in rodent T cells.
From: DPP8/9 inhibitors activate the CARD8 inflammasome in resting lymphocytes
The indicated mouse, rat, and human (Donor 011) CD3+ T cells were treated with VbP or 8j for 24 h before cell viability was assessed by Cell-TiterGlo (a) and lysates were evaluated by immunoblotting (b, c). d C57BL/6 BMDMs, BALB/c BMDMs, SD BMDMs, LEW BMDMs, mouse RAW 264.7 cells, and human MV4;11 cells were treated with the indicated concentration of VbP for 24 h before cell viability was assessed by Cell-TiterGlo. Data are means ± s.e.m. of four or five biological replicates. ***p < 0.001, **p < 0.01 by two-sided Student’s t test. NS not significant. e BALB/c CD3+ T cells and BMDMs were either unprimed or primed with LPS (12 h) before being treated with VbP (6 h) methyl bestatin (Me-Bs, 6 h), LT (6 h), Lfn-Flagellin (3 h), or nigericin (1 h). Cell lysates and culture supernatants as indicated were evaluated by immunoblotting.
