Fig. 2: Increased macrophage-mediated apoptotic cell clearance in the absence of glial specification. | Cell Death & Disease

Fig. 2: Increased macrophage-mediated apoptotic cell clearance in the absence of glial specification.

From: Overexposure to apoptosis via disrupted glial specification perturbs Drosophila macrophage function and reveals roles of the CNS during injury

Fig. 2

a, b Ventral views of stage 15 control (a) and repo mutant (b) embryos containing GFP and red stinger-expressing macrophages; lower panels show zooms of macrophages indicated by boxes in upper panels. c Scattergraph of vacuoles per macrophage per embryo from genotypes in (a, b); P < 0.0001 via Mann–Whitney test (n = 24 and 30). d, e Ventral views of stage 15 control (d) and repo mutant (e) embryos containing GFP-labelled macrophages immunostained for GFP (green in merge) and apoptotic cells (anti-cDCP-1, magenta in merge); zooms show macrophages indicated in boxed regions. f Scattergraphs of phagocytic index (cDCP-1 puncta per macrophage per embryo); P < 0.0001 (n = 101 and 69 cells from 6 and 5 embryos, respectively) and P = 0.0043 (n = 6 and 5 embryos, respectively) via Mann–Whitney tests. Scale bars represent 20 μm or 5 μm in zooms; error bars and lines show standard deviation and mean, respectively (c, f); genotypes are w;srp-GAL4,UAS-GFP/srp-GAL4,UAS-red stinger (a, c), w;srp-GAL4,UAS-GFP/srp-GAL4,UAS-red stinger;repo03702 (b, c), w;;crq-GAL4,UAS-GFP (d, f) and w;;repo03702,crq-GAL4,UAS-GFP (e, f).

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