Fig. 7: Defective calcium responses to wounding in repo mutants are restricted to less superficial tissues.

a, b Calcium levels imaged via ubiquitous expression of GCaMP6M (da-GAL4,UAS-GCaMP6M) on wounding of the ventral surface of control (a) and repo mutant (b) stage 15 embryos; images show pre-wound calcium levels, immediately after wounding (0 min) and 5 min after wounding. Dotted lines show edges of embryo and wound edges in pre-wound and 5 min images, respectively. c Scattergraph showing area of GCaMP6M response immediately after wounding; Mann–Whitney test used to compare control vs repo (n = 35 and 47, respectively; P = 0.66). d Scattergraph showing ratio of GCaMP6M intensity in wound area before and immediately after wounding; Mann–Whitney test used to compare control vs repo (n = 23 and 26, respectively; P = 0.22). e–g maximum projections of superficial/epithelial regions (0–5 μm, e), superficial half of the VNC (10–20 μm from surface, f) and deeper/dorsal half of the VNC (20–30 μm from the surface, g) of the ventral side of a wounded control embryo containing ubiquitous expression of GCaMP6M; panels show pre-wound calcium levels, immediately after wounding (0 min) and 5 min after wounding. Dotted lines show edges of epithelial wound at 5 min, solid circles show physical damage at deeper regions of the embryo; embryo has not been orientated in order to show larger region of the response to wounding. Lines and error bars in scattergraphs show mean and standard deviation, respectively; scale bars represent 20 μm; ns indicates not significant (P > 0.05). Genotypes are w;;da-GAL4,UAS-GCaMP6M (control) and w;;repo⁰³⁷⁰²,da-GAL4,UAS-GCaMP6M (repo).