Fig. 6: UCHL1 interacts with and stabilizes CD36 protein.
From: Deubiquitination of CD36 by UCHL1 promotes foam cell formation
a Immunoprecipitated with CD36 antibody beads for UCHL1, b immunoprecipitated with UCHL1 antibody beads for CD36 protein expression. c Peritoneal macrophages (pMΦ) were transfected with MYC-tagged UCHL1 for 48 h and incubated with MYC and CD36 antibodies. Confocal microscopy was used to test the location of MYC and CD36. d, e Macrophages were treated with LDN57444 for 24 h. Before harvested 6 h, MG132 were used to treated cells. f Macrophages were transfected with UCHL1 siRNA for 48 h and MG132 for 6 h. Western blot and Co-IP assays for polyubiquitin and K48-polyubiquitin expression. g HEK293T cells were transfected with FLAG-CD36, His-UCHL1/WT, His-UCHL1/C90S, and HA-Ub. Immunoprecipitated with FLAG and then immunoblotted with HA.
