Fig. 7: miR-450b-5p inhibition leads to fibrogenesis by regulating Runx1. | Cell Death & Disease

Fig. 7: miR-450b-5p inhibition leads to fibrogenesis by regulating Runx1.

From: LncRNA Hoxaas3 promotes lung fibroblast activation and fibrosis by targeting miR-450b-5p to regulate Runx1

Fig. 7

a, b qPCR and western blot analysis of Runx1 mRNA (a) and protein (b) level in lung fibroblasts after transfection of miR-450b-5p inhibitor together with Runx1 inhibition. c–e qPCR analysis of mRNA levels of collagen 1a1 (c), collagen 3a1 (d), and Fibronectin (e). f Western blot analysis of expression of Fibronectin, Vimentin, and a-SMA. (g) Proliferative capacity was analyzed using an EdU assay. h Wound-healing assays were performed to examine cell migration. (All data are presented as mean ± SD, n = 3, *P < 0.05).

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