Fig. 1: LncRNA-TTN-AS1 regulated TTN expression in SKCM cells. | Cell Death & Disease

Fig. 1: LncRNA-TTN-AS1 regulated TTN expression in SKCM cells.

From: Long noncoding RNA TTN-AS1 facilitates tumorigenesis and metastasis by maintaining TTN expression in skin cutaneous melanoma

Fig. 1

a, b Kaplan–Meier analysis of overall survival in SKCM case with high versus low TTN expression (n = 198) (a), and lncRNA-TTN-AS1 expression (n = 165) (b). c Significant correlation between lncRNA-TTN-AS1 and TTN expression in seven SKCM cell lines was calculated with Spearman test. d, e LncRNA-TTN-AS1 and TTN expression in B16F10 cells with downregulation (d) or overexpression (e) of lncRNA-TTN-AS1 were detected by qRT-PCR. f, g TTN protein level in B16F10 cells with downregulation (f) or overexpression (g) of lncRNA-TTN-AS1 was analyzed by western blotting, and β-actin was used as a loading control. h Subcellular localization of TTN and lncRNA-TTN-AS1 in B16F10 cell lines were measured by nuclear/cytoplasmic fractionation assays. Experiments were performed three times and data were presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Student t test.

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