Fig. 5: ADSCs treatment promotes the emigration of CXCR4high neutrophils independent of the CXCL12. | Cell Death & Disease

Fig. 5: ADSCs treatment promotes the emigration of CXCR4high neutrophils independent of the CXCL12.

From: Adipose-derived mesenchymal stromal cells promote corneal wound healing by accelerating the clearance of neutrophils in cornea

Fig. 5: ADSCs treatment promotes the emigration of CXCR4high neutrophils independent of the CXCL12.The alternative text for this image may have been generated using AI.

a–g Mice were treated as described in Fig. 1. a The absolute numbers of total and CXCR4high neutrophils in eyeballs were detected by flow cytometry on Day 3 (n = 10–11 mice/group). b, c Mice with ethanol injured eye were treated with/without AMD3100 and ADSCs, the absolute numbers of total and CXCR4high neutrophils (left b and c) in eyeballs were detected by flow cytometry on Day 7. And the vascular areas (right b) were also measured on Day 7 after AMD3100 treatment (n = 8 mice/group in B, n = 14–15 mice/group in (c). d The absolute numbers of total and CXCR4high neutrophils in lungs were detected by flow cytometry on Day 7 (n = 8 mice/group). e The mRNA levels of Cxcl-12 in the eyeball were determined by qPCR normalized to β-actin (n = 10 mice/group) on Day 3. f, g The protein levels of Cxcl-12 in the lung explant supernatant and serum were determined by ELISA (n = 12 mice/group) on Day 3. Data are shown as means ± SEM, *P < 0.05; ***P < 0.001; ****P < 0.0001 determined by one-way ANOVA with Tukey comparisons.

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