Fig. 6: ADSCs accelerate neutrophil reverse transmigration by regulating the expression of Jam-c in endothelial cells. | Cell Death & Disease

Fig. 6: ADSCs accelerate neutrophil reverse transmigration by regulating the expression of Jam-c in endothelial cells.

From: Adipose-derived mesenchymal stromal cells promote corneal wound healing by accelerating the clearance of neutrophils in cornea

Fig. 6: ADSCs accelerate neutrophil reverse transmigration by regulating the expression of Jam-c in endothelial cells.The alternative text for this image may have been generated using AI.

a–d Mice were treated as described in Fig. 1. a, b The mRNA levels of Jam-c, Ltb4, CD99, and Pecam1 in the eyeball were determined by qPCR normalized to β-actin (n = 10 mice/group) on Day 3. c, d The absolute numbers of CXCR4high neutrophils CXCR4high ICAM-1+CXCR1low and in lungs were detected by flow cytometry on Day 7 (n = 8 mice/group). e The mRNA levels of CD99, ICAM-1 and Jam-c in the HUVEC were determined by qPCR normalized to β-actin after co-cultured with medium or ADSCs supernatant. Data are shown as mean ± SEM of four replicates and representative of two independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 determined by one-way ANOVA with Tukey comparisons or t test.

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