Fig. 2: PIK3R3 regulates p21 expression in the p53-dependent way.
From: PIK3R3 inhibits cell senescence through p53/p21 signaling
a Western blot (WB) of PIK3R3, p53, and p21 both in LoVo and SW48 cells transfected with siNC, siPIK3R3(#1,#2). b qPCR assays of PIK3R3, p53, and p21 mRNA in LoVo (up panel) and SW48 (down panel) cells transfected with siNC, siPIK3R3 (#1,#2). These data were normalized to corresponding GAPDH. c LoVo cells transfected with siNC or siPIK3R3#1 were treated with CHX(100 μM) in different time lengths (0, 0.5, 1, 2, and 4 h). WB showed the change trend of p21 protein over time. d Luciferase activities of p21 promotor in LoVo (left panel) and SW48 (right panel) cells transfected with p21-luc and siNC, siPIK3R3(#1,#2). e, f WB of PIK3R3, p53, and p21 in HCT116 p53−/− cells transfected with siNC, siPIK3R3 (#1, #2), or with PIK3R3-expressing plasmid. Every assay repeated three times, and these numeral results were displayed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, t-test.
