Fig. 2: miR-133a promotes the differentiation of C2C12 myoblasts. | Cell Death & Disease

Fig. 2: miR-133a promotes the differentiation of C2C12 myoblasts.

From: KAP1-associated transcriptional inhibitory complex regulates C2C12 myoblasts differentiation and mitochondrial biogenesis via miR-133a repression

Fig. 2

a PCR quantification of miR-133a during cell differentiation. RNU6B was used as the control. Data are presented as the mean ± SEM (n = 5); **P < 0.01. b The transfection efficacy of miR-133a mimic. Intracellular miR-133a level was detected by RT-PCR after transfection with miR-133a mimic for 72 h. Data are presented as the mean ± SEM (n = 3); **P < 0.01. c RT-PCR quantification of differentiation marker Myhc, Myod1, Myog, and Ckm. Data are presented as the mean ± SEM (n = 4); *P < 0.05; **P < 0.01. d The influence of miR-133a on the number of MYHC-positive cells. C2C12 cells were transfected with miR-133a mimic or miR-133a inhibitor for 72 h and then stained for MYHC and DAPI. Fluorescence images were obtained with FV1000 microscope, scale bar = 100 μm. e The influence of miR-133a on the horse serum-induced differentiation of C2C12 cells. Cells were transfected with miR-133a mimic or inhibitor for 72 h and then induced by 2% horse serum for 4 days, stained for MYHC and DAPI, and fluorescence images were obtained with FV1000. Scale bar: 100 μm (up) and 50 μm (down). Data are presented as the mean ± SEM (n ≥ 12), *P < 0.05, **P < 0.01.

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