Fig. 1: Plasma exosomal miRNAs level in GC patients.

Exosomes were isolated from plasma samples of 87 GC patients by stepwise centrifugation. The isolation of exosomes was confirmed by detecting CD63 and CD81 by immunoblotting (a). b Electronic microscopic image of exosomes. c Total RNAs were extracted by Trizol reagents and miRNAs level were determined by qRT-PCR. Results were analyzed by student’s t-test and P < 0.05 was considered statistically significant.