Fig. 2: Impact of heme iron and inorganic iron on cell and intestinal organoid viability. | Cell Death & Disease

Fig. 2: Impact of heme iron and inorganic iron on cell and intestinal organoid viability.

From: Heme oxygenase 1 protects human colonocytes against ROS formation, oxidative DNA damage and cytotoxicity induced by heme iron, but not inorganic iron

Fig. 2: Impact of heme iron and inorganic iron on cell and intestinal organoid viability.The alternative text for this image may have been generated using AI.

a–c HCEC (a), HCT116 (b) and Caco-2 (c) were treated with increasing concentrations of hemin or FeCl3 (0–200 µM). Cell viability was determined after 72 h using the MTS assay. Data are given as mean + SEM (n ≥ 3, triplicates). Ns: p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (versus respective control). d Microscopic images of isolated intestinal crypts (day 0) and the developing intestinal organoids (day 1 and 4). e Intestinal organoids were treated with hemin or FeCl3 for 24 h and viability was determined by the MTT assay. Date are given as mean + SEM (n = 2). Ns: p > 0.05; **p < 0.01; ****p < 0.0001.

Back to article page