Fig. 3: Uptake of hemin and inorganic iron into HCEC and CRC cells. | Cell Death & Disease

Fig. 3: Uptake of hemin and inorganic iron into HCEC and CRC cells.

From: Heme oxygenase 1 protects human colonocytes against ROS formation, oxidative DNA damage and cytotoxicity induced by heme iron, but not inorganic iron

Fig. 3: Uptake of hemin and inorganic iron into HCEC and CRC cells.The alternative text for this image may have been generated using AI.

a HCEC were incubated with hemin or FeCl3 for different periods (0, 0.25, 8 and 24 h) at the concentrations indicated. Cells were then collected and iron content was determined by ICP-MS as described. Data are depicted as mean + SEM (n ≥ 3). b HCT116 cells were treated with hemin or FeCl3 and iron content was analyzed as mentioned above. Data are shown as mean + SEM (n = 7). c Caco-2 cells were treated and analyzed as described under a. Data are presented as mean + SEM (n ≥ 6). d HCEC and HCT116 cells were exposed to hemin or FeCl3 for 24 h. Cells were harvested, lysed and subjected to Western blot analysis for heme oxygenase-1 (HO-1) and ferritin heavy chain gene (FtH). Hsp90 was detected as loading control.

Back to article page