Fig. 4: Epigenomic analysis reveals force-induced dynamics of H3K27me3 landscape surrounding the differentially regulated mechanoresponsive genes.

a Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) using antibodies against H3K27me3 was performed in unstressed PDLSCs with two biological replicates. Aggregate plot for each of the four differentially regulated groups in addition to a control group with no significant expression change showed average H3K27me3 occupancy in a 1 million base window centered by transcription start sites (TSS) of downstream genes. rep, replicate. b ChIP-seq analysis using antibodies against H3K27me3 was also performed in the stressed PDLSCs. Empirical cumulative distribution function for the mechanical stress induced H3K27me3 binding differences within the central window enclosing TSS of the indicated differentially regulated sets of genes was plotted. c Aggregate plot showed the average H3K27ac and H3K27me3 occupancy along the 100 kb window centered by TSS of downstream genes from each of the five groups defined in a in the absence or presence of mechanical stress. Regions with detectable H3K27me3 occupancy changes were shaded. d ChIP-seq profiles along the EZH2 locus for input or the indicated histone modifications.