Fig. 5: Glycolysis stress test showed a decrease in glycolytic activity in FoxO1 conditional knockout macrophages. | Cell Death & Disease

Fig. 5: Glycolysis stress test showed a decrease in glycolytic activity in FoxO1 conditional knockout macrophages.

From: Multi-omics analysis identifies FoxO1 as a regulator of macrophage function through metabolic reprogramming

Fig. 5

a Kinetic ECAR response of FoxO1mKO (blue line) and control (red line) macrophages to glucose (10 mM), oligomycin (1.0 μM), and 2-DG (50 mM) (n ≥ 3). Macrophages were plated at 80,000 cells per well in XFe96 cell culture plates 1 day prior to the assays. b–d Statistical analysis of (b) non-glycolytic acidification, (c) glycolysis, and (d) glycolytic capacity in FoxO1 conditional knockout macrophages vs. control macrophages. e Luminescent signal of FoxO1mKO (blue), control (red) macrophages and cell counts. f Statistical analysis of ATP per cells of FoxO1mKO (blue), control (red) macrophages (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001.

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