Fig. 2: Differentiation of BAX/BAK DKO into hNPCs and validation of cell death phenotype.
From: Modeling the function of BAX and BAK in early human brain development using iPSC-derived systems
a Diagram of hiPSCs to hNPCs differentiation protocol based on inhibiting BMP and TGFB pathways. b Derived hNPCs were stained for neural progenitor markers PAX6, SOX2, and Nestin to validate. hiPSCs were also stained for PAX6 and SOX2 to validate. c hNPCs DKO clones have decreased cleaved caspase-3 activity due to loss of BAX and BAK with 24-h etoposide exposure. d hNPCs DKO clones demonstrate a decrease in CC3 compared to controls with 24-h CCCP exposure. Three independent experiments were conducted for etoposide and CCCP exposures. Error bars: SEM.
