Fig. 2: The effect of MTPα on insulin resistance. | Cell Death & Disease

Fig. 2: The effect of MTPα on insulin resistance.

From: SIRT1 deacetylates mitochondrial trifunctional enzyme α subunit to inhibit ubiquitylation and decrease insulin resistance

Fig. 2

a MTPα mRNA expression in TNF-α (4 ng/ml) treated 3T3-L1 adipocytes, WAT of HFD mice and db/db mice. b MTPα protein level in TNF-α (4 ng/ml) treated 3T3-L1 adipocytes. **p < 0.05 vs. control (t-test). c MTPα protein level in WAT of HFD-induced insulin-resistant mice. **p < 0.01 vs. chow fed mice (t-test). d MTPα protein level in WAT of db/db diabetic mice. *p < 0.05 vs. control mice (t-test). e MTPα protein levels as determined by western blotting in 3T3-L1 preadipocytes transfected with ovMTPα. **p < 0.01 vs. ovCON (t-test). f MTPα protein levels as determined by western blotting using 3T3-L1 preadipocytes transfected with shMTPα. *p < 0.05 vs. shCON (t-test). g Glucose transport in TNF-α treated 3T3-L1 adipocytes transfected with shMTPα or ovMTPα. Basal glucose transport (gray) and insulin stimulated glucose uptake (white) are shown. **p < 0.01, ****p < 0.0001 vs. TNF-α plus insulin (one-way ANOVA). h Insulin signaling examined by western blotting analysis in 3T3-L1 adipocytes. Cells treated with TNF-α (4 ng/ml) alone or together with ovMTPα for 4 days. Meanwhile all adipocytes were treated with insulin (5 μg/ml) for 4 days. *p < 0.05, **p < 0.01, ****p < 0.0001 vs. ovCON; #p < 0.05, ##p < 0.01 vs. ovCON+TNF (one-way ANOVA). i Insulin signaling examined by western blotting analysis in 3T3-L1 adipocytes. Cells treated with TNF-α (4 ng/ml) alone or together with shMTPα. Meanwhile all adipocytes were treated with insulin (5 μg/ml) for 4 days. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. shCON; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. shCON+TNF (one-way ANOVA). Data represent at least three different experiments. n = 5 mice per group. All data represent mean ± standard error (SE).

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