Fig. 1: OGD/R induces miR-422a elevation in neuronal cells. | Cell Death & Disease

Fig. 1: OGD/R induces miR-422a elevation in neuronal cells.

From: Oxygen glucose deprivation/re-oxygenation-induced neuronal cell death is associated with Lnc-D63785 m6A methylation and miR-422a accumulation

Fig. 1

SH-SY5Y cells (a–c) or primary murine cortical neurons (“neurons”, d–f) were maintained under oxygen glucose deprivation (OGD) for 4 h, followed by re-oxygenation (“OGD/R”) for the applied time; Expression of miR-422a and listed mRNAs was tested by qPCR assays (a, b, d and e); Listed proteins in total cell lysates were tested by Western blotting (c and f). The listed proteins were quantified and normalized to GAPDH (c and f). “Mock” stands for the mock treatment (norm-oxygenated medium with glucose) (Same for all Figures). Data indicate standard deviation (SD, n = 5). *p < 0.05 vs. “Mock” cells. Each experiment was repeated four times and similar results were obtained.

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