Fig. 5: Knockout of lncRNA Meg3 attenuates high glucose-induced mitochondrial fission and cell injury in human podocytes. | Cell Death & Disease

Fig. 5: Knockout of lncRNA Meg3 attenuates high glucose-induced mitochondrial fission and cell injury in human podocytes.

From: Increased long noncoding RNA maternally expressed gene 3 contributes to podocyte injury induced by high glucose through regulation of mitochondrial fission

Fig. 5

a Schematic diagram of target strategy of Meg3 knockout in human podocytes using the double-nickase/CRISPR-Cas9 System. Two pairs guide RNAs targeted to the promoter region and exon 3 of lncRNA Meg3, and the target sequence is approximately 3500 bp. b Expression of lncRNA Meg3 in human podocytes of different groups. c Expression of Nephrin and Synaptopodin in human podocytes of different groups. d Representative structured of mitochondria of human podocytes were stained by MitoTracker Deep Red and imaged by laser scanning confocal microscopy (original magnification, ×630; scale bar, 10 μm). e Quantitative analyses of expression of Nephrin and Synaptopodin in human podocytes of different groups. f Quantitative analyses of mitochondrial organization in human podocytes of different groups (mean branch length, mean number of branches per network, and mitochondrial footprint, n = 10 cells per group). Data were shown as mean ± SD in all statistical graphs. *P < 0.05, **P < 0.01, and ***P < 0.001 (n = 6 per group).

Back to article page