Fig. 7: LncRNA Meg3 promotes fission of the mitochondria in podocytes by regulation of Drp1 and its translocation to mitochondria. | Cell Death & Disease

Fig. 7: LncRNA Meg3 promotes fission of the mitochondria in podocytes by regulation of Drp1 and its translocation to mitochondria.

From: Increased long noncoding RNA maternally expressed gene 3 contributes to podocyte injury induced by high glucose through regulation of mitochondrial fission

Fig. 7

a, b Western blot analysis of proteins (Opa1, Mfn1, Mfn2 and Drp1) involved in the regulation of mitochondrial fission and fusion in podocytes from different groups. c–f Western blot analysis of pDrp1 (Ser637) in Meg3 KO and OE podocytes. g–i Western blot analysis of proteins in podocytes treated with HG or HG + Mdivi1 in Meg3 OE cell line. j Representative confocal images of mitochondria in human podocytes were stained by MitoTracker Deep Red and incubated with anti-Drp1 (original magnification, ×630; scale bar, 10 μm). k Quantitative analyses of mitochondrial organization on human podocytes from different groups (mean branch length, mean number of branches per network, and mitochondrial footprint, n = 10 cells per group). Data were shown as mean ± SD in all statistical graphs. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 6 per group), and n.s., not significant.

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