Fig. 3: Cytoplasmic LINC01106 has a positive effect on Gli4 expression by acting as a sponge for miR-449b-5p. | Cell Death & Disease

Fig. 3: Cytoplasmic LINC01106 has a positive effect on Gli4 expression by acting as a sponge for miR-449b-5p.

From: LINC01106 drives colorectal cancer growth and stemness through a positive feedback loop to regulate the Gli family factors

Fig. 3

a and b Luciferase activity of vector containing the whole length of LINC01106 in both LoVo and SW1116 transfected with miRNA mimics specific to 10 candidate miRNAs. c Three miRNAs were examined by qRT-PCR in normal cell line and dive CRC cells. d The binding sites of LINC01106 to miR-449b-5p were predicted. e Luciferase reporter assay introduced that activity of LINC01106-WT reporter or LINC01106-MUT reporter vectors in response to miR-449b-5p overexpression. f RIP assay elucidated that both LINC01106 and miR-449b-5p were enriched in RNA-induced silencing complex (RISC). g A positive correlation between LINC01106 and Gli4 expression was revealed in COAD tissues using GEPIA. h GEPIA showed that Gli4 had a close association with low overall survival rate of COAD patients. i Highly expressed Gli4 was found by TCGA related to advanced stages in COAD patients. j The sites in Gli4 3’UTR responsible for binding miR-499b-5p were shown and mutation at this region was performed. k Subsequent luciferase reporter assay verified that miR-499b-5p mimics declined the luciferase activity of Gli4-WT, whereas mutation at this place canceled this impact. l and m The mRNA and protein levels of Gli4 were measured in CRC cells transfected with sh-NC, sh-LINC01106, or co-transfected with sh-LINC01106 and miR-449b-5p inhibitors. Data shown as mean ± SD were collected from three independent experiments. **P < 0.01 indicated data had statistical significance.

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