Fig. 5: Knockdown of circRNA_09505 in macrophages prevented against arthritis in CIA mice. | Cell Death & Disease

Fig. 5: Knockdown of circRNA_09505 in macrophages prevented against arthritis in CIA mice.

From: CircRNA_09505 aggravates inflammation and joint damage in collagen-induced arthritis mice via miR-6089/AKT1/NF-κB axis

Fig. 5

a The flowchart for constructing CIA mice model (eight mice for each group). b Blocking circRNA_09505 in macrophages reduced the mean arthritis score and prolonged the time of arthritis that first appeared in contrast to sh-NC-macrophage-treated CIA mice. c Representative pictures of CIA mice model. Less severe joint redness and swelling in sh-circRNA-treated CIA mice compared with sh-NC group. d Representative HE staining of knee joint sections showed decreased subchondral bone erosions, damages, and lymphocyte infiltration in sh-circRNA-transfected macrophage-treated CIA mice. e Representative Safranin O staining presented less knee joint injury and bone destruction in sh-circRNA-macrophage-administered CIA mice. f Representative Toluidine blue staining of knee joint sections showed that knockdown of circRNA_09505 protected the CIA mice from severe bone destruction. g The quantitative analysis showed less severe synovitis and cartilage damage of CIA mice when circRNA_09505 was blocked in macrophages. Compared with controls, **P < 0.01, ***P < 0.001; in contrast to sh-NC group, ##P < 0.01. At least three repeated experiments were conducted.

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