Fig. 3: EFTUD2 expression is necessary for HCC cell survival. | Cell Death & Disease

Fig. 3: EFTUD2 expression is necessary for HCC cell survival.

From: EFTUD2 maintains the survival of tumor cells and promotes hepatocellular carcinoma progression via the activation of STAT3

Fig. 3

a The protein and mRNA levels of EFTUD2 expression in HCC cells detected by western blot and qPCR. b Western blot analysis of EFTUD2 expression in Hep3B and Huh7 cells infected with siRNA against EFTUD2 (siEFTUD2). c. d The effect of EFTUD2 on cellular viability was detected by CCK8 assays and colony formation assays, respectively. e Western blot analysis of EFTUD2 protein expression in Hep3B and Huh7 cells infected with lentiviruses expressing either scrambled shRNA or shRNA against EFTUD2 (shEFTUD2). f Representative images show the cell morphological changes after the stable knockdown of EFTUD2. Scale bars, 100 μm. g, h CCK8 assays and colony formation assays show the effect of EFTUD2 on cellular survival in Hep3B and Huh7 cells with a stable knockdown of EFTUD2. The data are presented as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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