Fig. 4: Depletion of EFTUD2 blocks cell cycle progression and promotes cell apoptosis. | Cell Death & Disease

Fig. 4: Depletion of EFTUD2 blocks cell cycle progression and promotes cell apoptosis.

From: EFTUD2 maintains the survival of tumor cells and promotes hepatocellular carcinoma progression via the activation of STAT3

Fig. 4

a EdU incorporation assays were used to identify the cells in S phase after EFTUD2 silencing in the indicated cells. Red: cell in S phase. Scale bars, 200 μm. b The cell cycle analyses were performed in Hep3B and Huh7 cells with EFTUD2 knockdown. c, d Fluorescence microscopy and flow cytometry were performed to captured early apoptotic cells by mitochondrial membrane potential changes in Hep3B and Huh7 cells with EFTUD2 knockdown. Red, JC-1 aggregates; green, JC-1 monomers. Scale bars, 10 μm. e, f EFTUD2-silenced Hep3B and Huh7 cells treated with or without CDDP (2 μg/mL; 48 h) were stained with a combination of Annexin V-FITC and PI and analyzed by FACS. Cells positive for FITC staining were counted as apoptotic cells. The bar graphs show the percentage of apoptotic cells. g Apoptosis-associated proteins of the indicated cells were measured by western blotting. The data are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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