Fig. 1: B7-H3 confers to radioresistance of colorectal cells in vitro. | Cell Death & Disease

Fig. 1: B7-H3 confers to radioresistance of colorectal cells in vitro.

From: B7-H3 regulates KIF15-activated ERK1/2 pathway and contributes to radioresistance in colorectal cancer

Fig. 1

a RT-qPCR analysis was used to determine the mRNA level of B7-H3 in HCT116 and RKO cells after 0, 2, 4, 6 or 8 Gy X-ray irradiation. b Western blot analysis was used to determine the protein level of B7-H3 in HCT116 and RKO CRC cells after 0, 2, 4, 6 or 8 Gy X-ray irradiation. β-actin served as an internal control. Values are expressed as the mean ± SD. c Colony formation assay of B7-H3-overexpressing HCT116 or RKO cells exposed to 0, 2, 4, 6 or 8 Gy X-ray irradiation. Up panel: Representative colonies from each group after X-rays irradiation. Down panel: Clonogenic cell survival curves were generated for B7-H3-overexpressing HCT116 and RKO cells after 0, 2, 4, 6 or 8 Gy irradiation. The sensitizer enhancement ratio (SER) was measured using the multitarget, single-hit model. D0, Dq and the calculated SER values of the corresponding groups are shown. d, e Viability of B7-H3-overexpressing HCT116 and RKO cells d or B7-H3-knockdown HCT116 and RKO cells e after 4 Gy X-ray irradiation was assessed with CCK8 assays. Values are expressed as the mean ± SD. (n = 5). **P < 0.01, *P < 0.05.

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