Fig. 2: B7-H3 reduces S phase arrest and cell apoptosis of colorectal cells. | Cell Death & Disease

Fig. 2: B7-H3 reduces S phase arrest and cell apoptosis of colorectal cells.

From: B7-H3 regulates KIF15-activated ERK1/2 pathway and contributes to radioresistance in colorectal cancer

Fig. 2

a, b The effect of B7-H3 overexpression (a) and B7-H3 knockdown (b) on the cell cycle distribution in HCT116 and RKO cells after exposure to 4 Gy X-ray irradiation. c, d Apoptosis was measured using Annexin V/7-AAD double staining in B7-H3-overexpressing (c) or B7-H3-knockdown (d) CRC cells after exposure to 4 Gy X-ray irradiation. The data are shown as the mean ± SD of three independent experiments. e, f The protein expression of Bcl-2, Bax and cleaved-caspase 3 in B7-H3-overexpressing (e) or B7-H3-knockdown (f) CRC cells after exposure to 4 Gy X-ray irradiation. β-actin served as a loading control. **P < 0.01, *P < 0.05.

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