Fig. 1: Assesment of pancreatic autophagy and endosomal function after LPS injection.
From: Impaired autophagy increases susceptibility to endotoxin-induced chronic pancreatitis
a Representative PCR agarose gel electrophorese image of PCR products obtained from the DNA of mice with the indicated genotypes. PCR fragments for Atg7F/F mice: 500bp (Atg7), blank (Cre); Atg7+/− mice: 1.5 Kb and 500bp (Atg7), 350bp (Cre); Atg7Δpan mice: 500bp (Atg7), 350bp (Cre). Evaluation of autophagic activity after LPS injection. b Representative autophagy immunoblots detecting LC3, p62, and ERK1/2 (loading control) in the pancreas of Atg7F/F and Atg7Δpan mice. c The normalized values of LC3-II, LC3-II/LC3-I, and p62 were quantified as means ± SEM of 4 animals per group. d Increased recycling endosome Rab11A after LPS. Representative immunoblots of Rab7, Rab5, Rab11A and ERK1/2 (loading control) in the pancreas of Atg7F/F and Atg7Δpan mice. e The normalized values of Rab7, Rab5, and Rab11A were quantified as means ± SEM of 4 animals per group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
