Fig. 1: MCL1 interacts with native p73 isoforms.
From: MCL1 binds and negatively regulates the transcriptional function of tumor suppressor p73
a MCL1 and either FLAG-TAp73α or a FLAG control were transiently overexpressed in HEK293T cells. Reciprocal co-immunoprecipatations were performed with either anti-MCL1 captured by Protein G dynabeads or with pre-conjugated anti-FLAG magnetic beads. Western blot analysis was used to confirm protein overexpression and reciprocal pulldown of full length p73 or MCL1 protein. b Visual representation of the domain structure of the native p73 constructs. c MCL1 and either FLAG-ΔNp73, FLAG-p73β, or a FLAG control were transiently overexpressed in HEK293T cells. MCL1 was immunoprecipitated and western blots were analyzed for either the FLAG tag or MCL1. Only lanes expressing FLAG-p73β and FLAG-ΔNp73 co-immunoprecipiated with MCL1.
