Fig. 3: GATA6 upregulates MUC1 expression by binding to both 1584 and 1456 GATA motifs in the promoter and enhancing its transcription. | Cell Death & Disease

Fig. 3: GATA6 upregulates MUC1 expression by binding to both 1584 and 1456 GATA motifs in the promoter and enhancing its transcription.

From: GATA6 promotes epithelial-mesenchymal transition and metastasis through MUC1/β-catenin pathway in cholangiocarcinoma

Fig. 3

a Impact of GATA6 knockdown on MUC1 promoter activity in QBC939 cells by luciferase reporter analyses. b Impact of GATA6 overexpression on MUC1 promoter activity in RBE cells by luciferase reporter analyses. c Bioinformatic data show two GATA motifs (1584 and 1456) in MUC1 promoter region. d, e GATA6 bound to MUC1 promoter in QBC939 and RBE cells by ChIP analyses. f Impact of 1584 or (and) 1456 GATA motif mutation on GATA6 regulating MUC1 promoter activity in QBC939 cells. g Impact of 1584 or (and) 1456 GATA motif mutation on GATA6 overexpression regulating MUC1 promoter activity in RBE cells. Control: cells transfected with empty lentivirus vectors. shGATA6: cells transfected with lentivirus vectors encoding short hairpin RNA targeting human GATA6. exGATA6: cells transfected with lentivirus vectors encoding human GATA6 CDS DNA. *P < 0.05.

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