Fig. 5: BMP4 Tg expression ameliorates maternal diabetes-induced vasculopathy. | Cell Death & Disease

Fig. 5: BMP4 Tg expression ameliorates maternal diabetes-induced vasculopathy.

From: Restoring BMP4 expression in vascular endothelial progenitors ameliorates maternal diabetes-induced apoptosis and neural tube defects

Fig. 5

a Blood vessel density in yolk sac and embryos. Scale bars = 1 mm. b The vascular endothelial cell marker CD31 staining in the yolk sac (Scale bar = 100 μm) and the embryo (Scale bar = 250 μm). The red arrow indicates NTDs. Quantification of blood vessel density in yolk sac was shown in the graphs. c HE staining shows blood islands. The red arrow indicates blood island. Scale bar = 100 μm. Quantification of blood island number per section was shown in the graph. d Immunofluorescent staining shows Flk-1+ progenitors (red dots) in yolk sac. Cell nuclei were stained with DAPI (blue). Scale bar = 100 μm. Quantification of Flk-1+ progenitors cell number per section was shown in the graph. Experiments were performed using three embryos from three different dams per group (n = 3); * indicates significant difference compared to the other groups (P < 0.05) in one-way ANOVA followed by Tukey tests. ND: nondiabetic; DM: diabetes mellitus.

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