Fig. 7: P73 downregulation greatly abolished inhibitory effects of BCL11B expression on cell stemness. | Cell Death & Disease

Fig. 7: P73 downregulation greatly abolished inhibitory effects of BCL11B expression on cell stemness.

From: BCL11B suppresses tumor progression and stem cell traits in hepatocellular carcinoma by restoring p53 signaling activity

Fig. 7

a mRNA expression levels of CSC-related markers and cell differentiation-related markers were analyzed in P73-silenced HCC cell lines. b Protein expression levels of CSC-related markers and cell differentiation-related markers were analyzed in P73-silenced HCC cell lines. c Effects of P73 silencing on CSC traits were assessed by sphere-forming assays in HepG2 cells and BCL11B-OE Huh7 cells. d Self-renewal ability of P73-silenced HepG2 cells and BCL11B-OE Huh7 cells was evaluated by in vitro serial sphere-forming assays. e Cell apoptosis rates of P73-silenced HepG2 cells and BCL11B-OE Huh7 cells were evaluated by flow cytometry after 5 μM sorafenib or 2 μM doxorubicin treatment. Error bars represent the standard deviation (SD) from at least three independent experiments; *,P < 0.05; **P < 0.01; ***P < 0.001.

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