Fig. 4: CLCF1 is targeted directly by miR-30a-5p. | Cell Death & Disease

Fig. 4: CLCF1 is targeted directly by miR-30a-5p.

From: RETRACTED ARTICLE: The miR-30a-5p/CLCF1 axis regulates sorafenib resistance and aerobic glycolysis in hepatocellular carcinoma

Fig. 4

a Prediction of CLCF1 as a target of miR-30a-5p. miR-30a-5p binding site, as predicted in CLCF1 3′-UTR. b Assay using dual-luciferase reporters. Normalization of activity of renilla luciferase was done with firefly luciferase activity. c, d CLCF1 expression in miR-30a-5p mimic-transfected HepG2-S-R cells was assessed through real-time PCR or Western blotting. e, f CLCF1 expression in miR-30a-5p inhibitor-transfected HepG2 cells transfected with anti-miR-30a-5p was analyzed by real-time PCR and Western blotting. All data are mean ± SD of three separate experiments. ** P < 0.01.

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