Fig. 5: MiR-192/215-SET8 axis induces downregulation of senescence-associated secretory factors. | Cell Death & Disease

Fig. 5: MiR-192/215-SET8 axis induces downregulation of senescence-associated secretory factors.

From: Histone methyltransferase SET8 is regulated by miR-192/215 and induces oncogene-induced senescence via p53-dependent DNA damage in human gastric carcinoma cells

Fig. 5

A, B Validation of the expression of SASP, including p53, p21, IL-1a, IL-1b, IL-6 and IL-8. C Cells fixed and incubated with a SA-β-Gal staining solution. Cells imaged for SA-β-Gal. Percentage of SA-β-Gal-positive cells was determined for a minimum of 5 low-power magnification fields. The bar graph on the bottom shows the proportion of SA-β-Gal-positive cells (scale bar, 50 µm). 192, miR-192; 215, miR-215; inh, inhibitor; si, siRNA. *P < 0.05.

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