Fig. 7: PDT treatment triggered autophagy by activating the ROS/JNK signaling pathway and inhibiting the mTOR signaling pathway. | Cell Death & Disease

Fig. 7: PDT treatment triggered autophagy by activating the ROS/JNK signaling pathway and inhibiting the mTOR signaling pathway.

From: Photodynamic therapy induces autophagy-mediated cell death in human colorectal cancer cells via activation of the ROS/JNK signaling pathway

Fig. 7

A HCT116 and B SW480 cells were treated with 0.7 μmol/L m-THPC and 5 mmol/L NAC for 8 h, and then irradiated with a light dose of 3 J/cm2, followed by incubation without irradiation for another 12 or 24 h. Western blot analysis of p-JNK (Thr183/Tyr185) and JNK levels in HCT116 and SW480 cells. HCT116 and SW480 cells were treated with C 0.7 μmol/L m-THPC or D 0.35 μmol/L VP in the presence or absence of 10 μmol/L SP600125 for 8 h, and then irradiated with a light dose of 3 J/cm2, followed by incubation without irradiation for 16 h. Western blot analysis of p-JNK, JNK, MAP1LC3B-I, and MAP1LC3B-II levels in HCT116 and SW480 cells. E, F Flow cytometry was applied to measure cell apoptosis. G, H HCT116 and SW480 cells were treated with 0.7 μmol/L m-THPC or 0.35 μmol/L VP, respectively, for 8 h, and then irradiated with a light dose of 3 J/cm2, followed by incubation without irradiation for 8 h. Western blot analysis of p-p70S6K (Thr389), p70S6K, p-mTOR (Ser2481), p-mTOR (Ser2448), mTOR, MAP1LC3B-I, MAP1LC3B-II, and SQSTM1/p62 levels in cells. All data are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01.

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