Fig. 4: Mitochondrial cristae dynamics is regulated by OPA1, Yme1L, MICOS complex, and ATAD3A.

A Representative Hessian-SIM images of mitochondria in Live WT and OPA1 knockout (KO) MEFs stained with MitoTracker Green (250 nM, 15 min). B Live WT and OPA1 KO MEFs were stained with MitoTracker Green (250 nM, 15 min), and tracked and imaged by time-laps Hessian-SIM. Mitochondrial cristae dynamic events, including shortening, elongation, crista–IBM contact, crista–crista contact, and crista detaching from IBM were quantified (three independent experiments, about 100 mitochondria cristae for each experiment, total mitochondria cristae were indicated). Statistical significance was assessed from the student’s t-test; error bars indicate the means ± SD of three independent experiments, N.S. indicates “not significant”, ***p < 0.001 versus control. C Representative TEM images of mitochondria in WT and OPA1 KO MEFs. D Representative Hessian-SIM images of mitochondria in live control and Yme1L KO HCT116 cells stained with MitoTracker Green (250 nM, 15 min). E Live control and Yme1L KO HCT116 cells were stained with MitoTracker Green (250 nM, 15 min), and tracked and imaged by time-laps Hessian-SIM. Mitochondrial cristae dynamic events, including shortening, elongation, crista–IBM contact, crista–crista contact, and crista detaching from IBM were quantified (three independent experiments, about 100 mitochondria cristae for each experiment, total mitochondria cristae were indicated). Statistical significance was assessed from the student’s t-test; error bars indicate the means ± SD of three independent experiments, N.S. indicates “not significant”, *p < 0.05, ***p < 0.001 versus control. F Representative TEM images of mitochondria in control and Yme1L KO HCT116 cells. G Representative TEM images of mitochondria in control, Mic10 KO, Mic19 KO, Mic60 knockdown (KD), Sam50 KD, and ATAD3A KO HeLa cells. Magnified images in the boxed regions are shown as insets. H Live control, Mic10 KO, Mic19 KO, Mic60 KD, Sam50 KD, and ATAD3A KO HeLa cells were stained with MitoTracker Green (250 nM, 15 min) and imaged by Hessian-SIM. The representative mitochondrial cristae were displayed. I Live control, Mic10 KO, Mic19 KO, Mic60 KD, Sam50 KD, and ATAD3A KO HeLa cells were stained with MitoTracker Green (250 nM, 15 min), and tracked and imaged by time-laps Hessian-SIM. Mitochondrial cristae dynamic events, including shortening, elongation, crista–IBM contact, crista–crista contact, and crista detaching from IBM were quantified (three independent experiments, about 100 mitochondria cristae for each experiment, total mitochondria cristae were indicated). Statistical significance was assessed from the student’s t-test; error bars indicate the means ± SD of three independent experiments, **p < 0.01, ***p < 0.001 versus control.