Fig. 5: PDK4-mediated mitochondria-associated endoplasmic reticulum membrane disruption correlates with abnormal mitochondrial respiratory capacity. | Cell Death & Disease

Fig. 5: PDK4-mediated mitochondria-associated endoplasmic reticulum membrane disruption correlates with abnormal mitochondrial respiratory capacity.

From: PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming

Fig. 5

a Representative images and quantitative analysis of ER/mitochondria interactions. The ER was stained with ER-Tracker Red, and the mitochondria was stained with MitoTracker Green and then imaged with confocal microscopy. Scale bars = 10 μm. N = 40–50 cells per group. b Representative TEM images (scale bar = 100 nm) of the VSMCs treated with 1 mM DCA in the presence or absence of β-GP. c Protein expression of FUNDC1, IP3R1, VDAC1, Mfn1 and Mfn2 was determined by western blotting. N = 3 independent experiments. d The OCR was measured by using an Agilent Seahorse extracellular flux analyser. *P < 0.05, **P < 0.01, ***P < 0.001.

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