Fig. 5: Increased Nox4 levels in Parp3-deficient astrocytes cause impaired activation of mTorc2. | Cell Death & Disease

Fig. 5: Increased Nox4 levels in Parp3-deficient astrocytes cause impaired activation of mTorc2.

From: Parp3 promotes astrocytic differentiation through a tight regulation of Nox4-induced ROS and mTorc2 activation

Fig. 5

a Western blot analysis for the levels of p-Akt (S473), Akt and β actin as loading control, p-Gsk3β (S9), Gsk3β and β actin as loading control, Rictor, p-mTOR(S2481), mTOR and β actin as loading control and GFAP relative to β actin as loading control in NPSCs and throughout astrocytes differentiation (d2-d10) in Parp3+/+ and Parp3–/– cultures. b Bar graphs depict the relative signal intensities of p-Akt versus Akt, p-Gsk3β versus Gsk3β, Rictor versus β actin and p-mTor versus mTor measured in three independent experiments and two independent clones using Image J. Mean values ± s.d. are indicated. *P < 0.05, **P < 0.01, ***P < 0.001. c The depletion of Nox4 re-establishes p-Akt(S473) expression in Parp3-deficient astrocytes. Western blot analysis for the expression of Nox4 versus β actin as loading control, p-Akt (S473) versus Akt and p-Gsk3β versus Gsk3β and β actin used as loading control in sicontrol (siCTL) and siNox4-treated Parp3+/+ and Parp3–/– NPSCs and astrocytes d4. d Bar graphs depict the relative signal intensities of p-Akt versus Akt, p-Gsk3β versus Gsk3β, measured in three independent experiments using Image J. Values represent means ± s.d. of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page