Fig. 4: ENO1 promotes HCC cell proliferation, migration and invasion and EMT in vitro.

A CCK-8 assays, B colony formation assays, C wound healing assays, D transwell migration and invasion assays of HCCLM3 cells after ENO1 knockdown. E Western blot analysis of E-cadherin, N-cadherin and Vimentin levels in HCCLM3 cells after ENO1 knockdown. f CCK-8 assays, G colony formation assays, H transwell migration and invasion assays of MHCC97L and HepG2 cells after ENO1 overexpression. I Western blot analysis of E-cadherin, N-cadherin and Vimentin levels in MHCC97L and HepG2 cells after ENO1 overexpression. A representative of three independent experiments is shown. All data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 according to two-tailed Student’s t-test.