Fig. 5: TRPV4 activated cytoskeleton and Rho protein pathway in EC. | Cell Death & Disease

Fig. 5: TRPV4 activated cytoskeleton and Rho protein pathway in EC.

From: Calcium and TRPV4 promote metastasis by regulating cytoskeleton through the RhoA/ROCK1 pathway in endometrial cancer

Fig. 5: TRPV4 activated cytoskeleton and Rho protein pathway in EC.The alternative text for this image may have been generated using AI.

a, b GSEA analysis demonstrated high expression of TRPV4 is enriched in actin filament organization and anchored component of plasma membrane. c The results of cytoskeleton assay of ishikawa cells visualized by confocal microscopy, with representative images shown. Cell nuclei were stained with DAPI. Magnification 63×. Scale bar: 25 or 10 μm. d Statistical numbers of actin filaments and PXN in each group. e TRPV4 silencing and the expression of Rho family GTPase pathway in ishikawa cell. f Relationship between calcium concentration and Rho family GTPase pathway. g Calcium chelating agent BAPTA-AM (10 μM) significantly inhibited the RhoA-ROCK1 pathway. Columns represent mean ± SEM. Numbers below the Western blot band, relative expression to GAPDH of each protein.

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