Fig. 6: RhoA-ROCK1 mediated cytoskeleton through LIMK-cofilin pathway.

a Influence of Y27632 (10 μM) on the F-actin and PXN. Magnification 63×. Scale bar: 25 or 10 μm. b Statistical number of F-actin and PXN. c Association between TRPV4 expression and the downstream of ROCK1, including LIMK, cofilin, MLC, MYPT. d TRPV4 affected the expression of the RhoA-ROCK1- LIMK-cofilin pathway and these effects were amplified by TRPV4 agonist GSK1016790A (50 nM) or decreased by TRPV4 antagonist HC067047 (10 μM). e The synthetic effect of calcium, TRPV4, and RhoA on the RhoA-ROCK1- LIMK-cofilin pathway, Y-26632, RhoA antagonist. Columns represent mean ± SEM. Numbers below the Western blot band, relative expression to GAPDH of each protein.