Fig. 5: TINCR promotes EGFR expression by acting as a ceRNA. | Cell Death & Disease

Fig. 5: TINCR promotes EGFR expression by acting as a ceRNA.

From: LncRNA TINCR favors tumorigenesis via STAT3–TINCR–EGFR-feedback loop by recruiting DNMT1 and acting as a competing endogenous RNA in human breast cancer

Fig. 5: TINCR promotes EGFR expression by acting as a ceRNA.

a Subcellular localization of TINCR in breast-cancer cell lines, assessed using nuclear/cytoplasmic extract isolation assay. b Overlapping miRNAs in transcriptome miRNA sequencing data from HMUCC cohort and TargetScan, RNA22, and starBase databases. Venn diagrams were generated using Venny online software (http://bioinfogp.cnb.csic.es/tools/ venny/). c qRT-PCR examination of the expression of miRNAs in UACC 812 cells transfected with a TINCR-specific siRNA or scrambled siRNA. d, e Expression of TINCR, EGFR mRNA (d), and EGFR protein (e) in UACC 812 and MDA-MB-231 cells treated with miR-503-5p mimic or inhibitor. f, g Complementarity between miR-503-5p seed sequence and 3ʹ-UTRs of TINCR (f) and EGFR (g) predicted through a computational and bioinformatics-based approach by using TargetScan and StarBase online databases. Watson–Crick complementarity is connected by “|.” Nucleotide-replacement mutations made to the genes are underlined. h, i Luciferase-reporter assay for assessing interactions between miR-503-5p and its binding sites or mutated binding sites in 3ʹ-UTRs of TINCR (h) and EGFR (i) in HEK293T cells. Data are presented as means from three independent experiments ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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