Fig. 5: TalaA accelerated ferroptosis in CRC cells by down-regulation of SLC7A11. | Cell Death & Disease

Fig. 5: TalaA accelerated ferroptosis in CRC cells by down-regulation of SLC7A11.

From: Discovery of a novel ferroptosis inducer-talaroconvolutin A—killing colorectal cancer cells in vitro and in vivo

Fig. 5

A SLC7A11 mRNA was decreased by TalaA dose-dependently; *p < 0.05, **p < 0.01, N = 3 independent repeats. B SLC7A11 protein level was decreased by TalaA in a dose-dependent manner. C The SLC7A11 protein level was increased by SLC7A11 overexpression plasmid (SLC7A11 OVX) transfection. D The relative cell activities of SLC7A11-overexpressed cells and control cells after treated by 5.0 μM TalaA. **p < 0.01, N = 3 independent repeats. E The mRNA expression was suppressed by SLC7A11-specific lenti-shRNA; **p < 0.01 versus shCon, N = 3 independent repeats. F The SLC7A11 protein level was decreased by lenti-shSLC7A11. G Total glutathione and reduced glutathione was decreased as SLC7A11 being knocked down; **p < 0.01, N = 3 independent repeats. H 5.0 μM TalaA induced slight cell membrane to get destroyed in wild type SW480 cells. However same concentration of TalaA induced strong membrane to get destroyed in SLC7A11 knocked down SW480 cells. The yellow arrows indicate membrane-damaged cells. I 5.0 μM TalaA-treated SLC7A11 knocked-down SW480 cells had lower cell activity than wild type SW480 with same concentration TalaA treatment; **p < 0.01, N = 3 independent repeats. J The scatter plot of TalaA inhibited cell growth. The blue points represented wild type SW480, and red squares represented SLC7A11 knocked down SW480 cells. For each concentration point, three repeats were performed. K The SLC7A11 knockdown SW480 and wild type SW480 were treated by 5.0 μM TalaA with or without Ferrostatin-1. The cell activity of SW480 cells was detected with CCK8 kit; **p < 0.01, N = 3 independent repeats.

Back to article page