Fig. 1: GARP is expressed on several bone sarcoma cancer cell lines and its silencing blocks their proliferation.
A GARP mRNA expression data from various cancer cell lines retrieved from the CCLE data base. The numbers in parentheses indicate the number of cell lines analysed/cancer type. RMA log2 mRNA expression values are shown as box plots and sorted by the median in descending order from left to right. Outliers are plotted as filled circles. B GARP mRNA expression in primary BM-MSCs and various cancer cell lines. Data are shown as mean (SD), relative to the GARP expression in BM-MSCs. C Summary of GARP expression (% in relation to isotype control) on primary BM-MSCs and selected bone sarcoma cell lines. Data are shown as mean (SD) of three individual stainings. D Silencing of GARP in BM-MSCs and the osteosarcoma cell lines G292, T1–73, and SAOS-2 inhibits their proliferation. Proliferation (cell index) of non-transduced (NT), LV-CTRL, GARPKO1, and GARPKO2 cells was measured in real-time during 7 days. Data are shown as mean (SD) of experimental duplicates. One representative experiment out of three is shown. E Graphs show the mean (SD) of the proliferation represented as cell index at 160 h divided by the corresponding cell index at 10 h of three individual experiments. * =P < 0.05; *** =P < 0.001. F Silencing of GARP in osteosarcoma cell lines induce cell death by apoptosis. Apoptosis was measured in NT, LV-CTRL, GARPKO1, and GARPKO2 G292 (left graph), T1–73 (middle graph), and SAOS-2 (right graph) cells by staining the cells with 7AAD/Annexin V and analysing the cells by flow cytometry. Data are shown as mean (SD) of three independent experiments. * =P < 0.05.
